59.6 Folate:Active B12

59.6.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

Prolactin
Chisq Df Pr(>Chisq)
(Intercept) 105.9654174 1 <0.0001 ***
folate_Active.B12 2.8507424 1 0.09 ns
GDM_new 1.1655961 1 0.3 ns
Study 6.9823448 1 0.008 **
GA_samp 66.5831228 1 <0.0001 ***
folate_Active.B12:GDM_new 0.0040185 1 0.9 ns
GDM_new:Study 0.9576216 1 0.3 ns
folate_Active.B12:Study 7.2599035 1 0.007 **
folate_Active.B12:GDM_new:Study 0.0013920 1 1.0 ns
HPL
Chisq Df Pr(>Chisq)
(Intercept) 13.9681088 1 0.0002 ***
folate_Active.B12 0.4096199 1 0.5 ns
GDM_new 0.0935974 1 0.8 ns
Study 14.4440520 1 0.0001 ***
GA_samp 1058.4296326 1 <0.0001 ***
folate_Active.B12:GDM_new 2.3157121 1 0.1 ns
GDM_new:Study 0.2657622 1 0.6 ns
folate_Active.B12:Study 5.2239565 1 0.02 *
folate_Active.B12:GDM_new:Study 4.8125063 1 0.03 *
GH2
Chisq Df Pr(>Chisq)
(Intercept) 190.2290308 1 <0.0001 ***
folate_Active.B12 1.0860066 1 0.3 ns
GDM_new 0.1049198 1 0.7 ns
Study 1.3273208 1 0.2 ns
GA_samp 492.6053979 1 <0.0001 ***
folate_Active.B12:GDM_new 1.2525421 1 0.3 ns
GDM_new:Study 0.1156740 1 0.7 ns
folate_Active.B12:Study 1.9208434 1 0.2 ns
folate_Active.B12:GDM_new:Study 0.8227386 1 0.4 ns
Insulin
model term df1 df2 F.ratio Chisq p.value
1 folate_Active.B12 1 Inf 4.471 4.471 0.03 *
5 GDM_new 1 Inf 27.005 27.005 <0.0001 ***
7 Study 1 Inf 9.213 9.213 0.002 **
8 GA_samp 1 Inf 0.005 0.005 0.9 ns
2 folate_Active.B12:GDM_new 1 Inf 0.518 0.518 0.5 ns
4 folate_Active.B12:Study 1 Inf 0.028 0.028 0.9 ns
6 GDM_new:Study 1 Inf 0.836 0.836 0.4 ns
3 folate_Active.B12:GDM_new:Study 1 Inf 1.318 1.318 0.3 ns
HOMA.IR
model term df1 df2 F.ratio Chisq p.value
1 folate_Active.B12 1 Inf 5.750 5.750 0.02 *
5 GDM_new 1 Inf 24.286 24.286 <0.0001 ***
7 Study 1 Inf 13.077 13.077 0.0003 ***
8 GA_samp 1 Inf 0.143 0.143 0.7 ns
2 folate_Active.B12:GDM_new 1 Inf 0.855 0.855 0.4 ns
4 folate_Active.B12:Study 1 Inf 0.001 0.001 1.0 ns
6 GDM_new:Study 1 Inf 0.717 0.717 0.4 ns
3 folate_Active.B12:GDM_new:Study 1 Inf 1.532 1.532 0.2 ns
GLUC3_mmol.L
model term df1 df2 F.ratio Chisq p.value
1 folate_Active.B12 1 Inf 4.993 4.993 0.03 *
5 GDM_new 1 Inf 3.118 3.118 0.08 ns
7 Study 1 Inf 23.055 23.055 <0.0001 ***
8 GA_samp 1 Inf 1.291 1.291 0.3 ns
2 folate_Active.B12:GDM_new 1 Inf 1.879 1.879 0.2 ns
4 folate_Active.B12:Study 1 Inf 0.387 0.387 0.5 ns
6 GDM_new:Study 1 Inf 1.832 1.832 0.2 ns
3 folate_Active.B12:GDM_new:Study 1 Inf 0.193 0.193 0.7 ns

59.6.2 Model summary

Ratio of Geometric means (95% CI)

Prolactin
Var Effect P
(Intercept) 2.88 (2.33, 3.42) <0.0001
folate_Active.B12 -0.00 (-0.00, 0.00) 0.08
GDM_newGDM -0.22 (-0.54, 0.10) 0.17
StudySTOP -0.29 (-0.50, -0.08) 0.007
GA_samp 0.14 (0.11, 0.17) <0.0001
folate_Active.B12:GDM_newGDM -0.00 (-0.00, 0.00) 0.95
GDM_newGDM:StudySTOP 0.23 (-0.09, 0.54) 0.16
folate_Active.B12:StudySTOP 0.00 (0.00, 0.00) 0.004
HPL
Var Effect P
(Intercept) 0.55 (0.26, 0.83) 0.0002
folate_Active.B12 0.00 (-0.00, 0.00) 0.52
GDM_newGDM -0.03 (-0.24, 0.17) 0.76
StudySTOP 0.22 (0.11, 0.33) 0.0002
GA_samp 0.30 (0.28, 0.31) <0.0001
folate_Active.B12:GDM_newGDM -0.00 (-0.00, 0.00) 0.13
GDM_newGDM:StudySTOP -0.06 (-0.29, 0.17) 0.61
folate_Active.B12:StudySTOP -0.00 (-0.00, -0.00) 0.02
folate_Active.B12:GDM_newGDM:StudySTOP 0.00 (0.00, 0.00) 0.03
GH2
Var Effect P
(Intercept) -2.63 (-3.00, -2.26) <0.0001
folate_Active.B12 -0.00 (-0.00, 0.00) 0.19
GDM_newGDM -0.12 (-0.34, 0.10) 0.27
StudySTOP -0.10 (-0.24, 0.04) 0.18
GA_samp 0.27 (0.24, 0.29) <0.0001
folate_Active.B12:GDM_newGDM -0.00 (-0.00, 0.00) 0.50
GDM_newGDM:StudySTOP 0.15 (-0.07, 0.37) 0.17
folate_Active.B12:StudySTOP 0.00 (-0.00, 0.00) 0.07
Insulin
Var Effect P
(Intercept) 2.76 (2.09, 3.44) <0.0001
folate_Active.B12 -0.00 (-0.00, 0.00) 0.09
GDM_newGDM 1.06 (0.59, 1.53) <0.0001
StudySTOP -0.37 (-0.63, -0.10) 0.006
GA_samp -0.00 (-0.04, 0.04) 0.96
folate_Active.B12:GDM_newGDM -0.00 (-0.00, 0.00) 0.38
GDM_newGDM:StudySTOP -0.44 (-0.90, 0.02) 0.06
folate_Active.B12:StudySTOP 0.00 (-0.00, 0.00) 0.39
HOMA.IR
Var Effect P
(Intercept) 1.46 (0.73, 2.18) <0.0001
folate_Active.B12 -0.00 (-0.00, 0.00) 0.08
GDM_newGDM 1.15 (0.61, 1.69) <0.0001
StudySTOP -0.53 (-0.81, -0.25) 0.0002
GA_samp -0.01 (-0.05, 0.04) 0.72
folate_Active.B12:GDM_newGDM -0.00 (-0.00, 0.00) 0.28
GDM_newGDM:StudySTOP -0.48 (-1.00, 0.05) 0.07
folate_Active.B12:StudySTOP 0.00 (-0.00, 0.00) 0.43
GLUC3_mmol.L
Var Effect P
(Intercept) 1.81 (1.65, 1.97) <0.0001
folate_Active.B12 -0.00 (-0.00, -0.00) 0.0006
GDM_newGDM 0.16 (-0.03, 0.34) 0.09
StudySTOP -0.16 (-0.21, -0.11) <0.0001
GA_samp -0.01 (-0.02, 0.00) 0.25
folate_Active.B12:GDM_newGDM -0.00 (-0.00, 0.00) 0.11
GDM_newGDM:StudySTOP -0.13 (-0.31, 0.05) 0.17
folate_Active.B12:StudySTOP 0.00 (-0.00, 0.00) 0.47

59.6.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Folate:Active B12 ratio and may be misleading when interaction term is involved.

Prolactin
Study GDM_new response SE df lower.CL upper.CL
SCOPE Uncomplicated 101.62816 9.314017 132.5548 84.77824 121.8271
STOP Uncomplicated 90.29880 7.578499 94.9709 76.44011 106.6701
SCOPE GDM 81.05772 13.016128 534.6951 59.12887 111.1192
STOP GDM 90.21198 9.467076 203.8058 73.35054 110.9494
HPL
Study GDM_new response SE df lower.CL upper.CL
SCOPE Uncomplicated 88.04922 3.026850 288.7021 82.28881 94.21288
STOP Uncomplicated 101.59394 2.988394 163.6881 95.86127 107.66944
SCOPE GDM 75.32856 5.693745 550.9688 64.93511 87.38558
STOP GDM 100.33245 4.462084 371.6288 91.93106 109.50163
GH2
Study GDM_new response SE df lower.CL upper.CL
SCOPE Uncomplicated 2.293818 0.0966457 343.5562 2.111390 2.492009
STOP Uncomplicated 2.235757 0.0771283 204.6502 2.088745 2.393116
SCOPE GDM 1.958147 0.1944361 557.8906 1.611167 2.379852
STOP GDM 2.228177 0.1214521 354.5442 2.001677 2.480307
Insulin
Study GDM_new response SE df asymp.LCL asymp.UCL
SCOPE Uncomplicated 14.52969 1.1290796 Inf 12.47702 16.92006
STOP Uncomplicated 10.62686 0.6103258 Inf 9.49551 11.89300
SCOPE GDM 39.13558 7.9435717 Inf 26.29048 58.25658
STOP GDM 18.48524 1.9016206 Inf 15.10984 22.61467
HOMA.IR
Study GDM_new response SE df asymp.LCL asymp.UCL
SCOPE Uncomplicated 3.552503 0.2995868 Inf 3.011283 4.190996
STOP Uncomplicated 2.206362 0.1346450 Inf 1.957634 2.486692
SCOPE GDM 10.223246 2.4037386 Inf 6.448374 16.207925
STOP GDM 3.937619 0.4329703 Inf 3.174226 4.884607
GLUC3_mmol.L
Study GDM_new response SE df asymp.LCL asymp.UCL
SCOPE Uncomplicated 5.541282 0.0858373 Inf 5.375572 5.712100
STOP Uncomplicated 4.764642 0.0648083 Inf 4.639298 4.893372
SCOPE GDM 6.267473 0.5260221 Inf 5.316822 7.388102
STOP GDM 4.746362 0.0798218 Inf 4.592464 4.905416

59.6.4 Comparisons

59.6.4.1 Within Groups

Effect for every 500 unit increase in Folate:Active B12 ratio.
Hormone Contrast Study GDM_new Ratio of Geometric means (95% CI) P-value
Prolactin folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE Uncomplicated 0.958 (0.913, 1.005) 0.08
Prolactin folate_Active.B121123.57 / folate_Active.B12623.57 STOP Uncomplicated 1.057 (1.006, 1.110) 0.03
Prolactin folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE GDM 0.955 (0.874, 1.045) 0.3
Prolactin folate_Active.B121123.57 / folate_Active.B12623.57 STOP GDM 1.054 (0.980, 1.133) 0.2
HPL folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE Uncomplicated 1.009 (0.983, 1.035) 0.5
HPL folate_Active.B121123.57 / folate_Active.B12623.57 STOP Uncomplicated 0.965 (0.939, 0.992) 0.01
HPL folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE GDM 0.939 (0.860, 1.026) 0.2
HPL folate_Active.B121123.57 / folate_Active.B12623.57 STOP GDM 1.010 (0.967, 1.056) 0.6
GH2 folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE Uncomplicated 0.978 (0.947, 1.011) 0.2
GH2 folate_Active.B121123.57 / folate_Active.B12623.57 STOP Uncomplicated 1.021 (0.986, 1.057) 0.2
GH2 folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE GDM 0.958 (0.899, 1.022) 0.2
GH2 folate_Active.B121123.57 / folate_Active.B12623.57 STOP GDM 1.000 (0.949, 1.055) 1.0
Insulin folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE Uncomplicated 0.958 (0.912, 1.007) 0.09
Insulin folate_Active.B121123.57 / folate_Active.B12623.57 STOP Uncomplicated 0.989 (0.934, 1.047) 0.7
Insulin folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE GDM 0.922 (0.847, 1.005) 0.06
Insulin folate_Active.B121123.57 / folate_Active.B12623.57 STOP GDM 0.952 (0.879, 1.031) 0.2
HOMA.IR folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE Uncomplicated 0.955 (0.907, 1.006) 0.08
HOMA.IR folate_Active.B121123.57 / folate_Active.B12623.57 STOP Uncomplicated 0.985 (0.926, 1.048) 0.6
HOMA.IR folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE GDM 0.908 (0.826, 0.998) 0.05
HOMA.IR folate_Active.B121123.57 / folate_Active.B12623.57 STOP GDM 0.936 (0.861, 1.018) 0.1
GLUC3_mmol.L folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE Uncomplicated 0.991 (0.986, 0.996) 0.0006
GLUC3_mmol.L folate_Active.B121123.57 / folate_Active.B12623.57 STOP Uncomplicated 0.996 (0.984, 1.008) 0.5
GLUC3_mmol.L folate_Active.B121123.57 / folate_Active.B12623.57 SCOPE GDM 0.971 (0.945, 0.998) 0.04
GLUC3_mmol.L folate_Active.B121123.57 / folate_Active.B12623.57 STOP GDM 0.976 (0.957, 0.995) 0.02

59.6.4.2 Between Groups

Below are comparisons of the differences in the change in Folate:Active B12 ratio between groups. I.e. whether the change in Folate:Active B12 ratio are different between groups.

Prolactin
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 1.2168295 0.0822859 683.0468 1.065532 1.389610 0.004 **
STOP/SCOPE (Uncomplicated) 1.1030999 0.0372976 683.0468 1.032246 1.178817 0.004 **
STOP/SCOPE (GDM) 1.1030999 0.0372976 683.0468 1.032246 1.178817 0.004 **
GDM/Uncomplicated (SCOPE) 0.9973718 0.0422328 642.2031 0.917795 1.083848 0.950 ns
GDM/Uncomplicated (STOP) 0.9973718 0.0422328 642.2031 0.917795 1.083848 0.950 ns
HPL
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 1.0293092 0.0555449 422.6078 0.9257213 1.1444885 0.593 ns
STOP/SCOPE (Uncomplicated) 0.9569665 0.0184170 709.0099 0.9214827 0.9938168 0.023 *
STOP/SCOPE (GDM) 1.0755958 0.0538283 407.0315 0.9748181 1.1867919 0.146 ns
GDM/Uncomplicated (SCOPE) 0.9313548 0.0435245 362.4851 0.8495777 1.0210034 0.129 ns
GDM/Uncomplicated (STOP) 1.0468090 0.0276512 716.9310 0.9939056 1.1025284 0.084 ns
GH2
contrast ratio SE df lower.CL upper.CL p.value
SCOPE/SCOPE (Overall) 1.0891701 0.0517284 662.4498 0.9921909 1.195628 0.073 ns
STOP/SCOPE (Uncomplicated) 1.0436331 0.0247828 662.4498 0.9960878 1.093448 0.073 ns
STOP/SCOPE (GDM) 1.0436331 0.0247828 662.4498 0.9960878 1.093448 0.073 ns
GDM/Uncomplicated (SCOPE) 0.9796986 0.0299631 662.5828 0.9225964 1.040335 0.503 ns
GDM/Uncomplicated (STOP) 0.9796986 0.0299631 662.5828 0.9225964 1.040335 0.503 ns
Insulin
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.0649411 0.0772902 Inf 0.9237363 1.227731 0.386 ns
STOP/SCOPE (Uncomplicated) 1.0319598 0.0374482 Inf 0.9611120 1.108030 0.386 ns
STOP/SCOPE (GDM) 1.0319598 0.0374482 Inf 0.9611120 1.108030 0.386 ns
GDM/Uncomplicated (SCOPE) 0.9623745 0.0419947 Inf 0.8834879 1.048305 0.379 ns
GDM/Uncomplicated (STOP) 0.9623745 0.0419947 Inf 0.8834879 1.048305 0.379 ns
HOMA.IR
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.0631318 0.0826268 Inf 0.9129177 1.238063 0.431 ns
STOP/SCOPE (Uncomplicated) 1.0310828 0.0400680 Inf 0.9554673 1.112683 0.431 ns
STOP/SCOPE (GDM) 1.0310828 0.0400680 Inf 0.9554673 1.112683 0.431 ns
GDM/Uncomplicated (SCOPE) 0.9506305 0.0447923 Inf 0.8667710 1.042603 0.283 ns
GDM/Uncomplicated (STOP) 0.9506305 0.0447923 Inf 0.8667710 1.042603 0.283 ns
GLUC3_mmol.L
contrast ratio SE df asymp.LCL asymp.UCL p.value
SCOPE/SCOPE (Overall) 1.0099613 0.0139150 Inf 0.9830534 1.037606 0.472 ns
STOP/SCOPE (Uncomplicated) 1.0049683 0.0069231 Inf 0.9914905 1.018629 0.472 ns
STOP/SCOPE (GDM) 1.0049683 0.0069231 Inf 0.9914905 1.018629 0.472 ns
GDM/Uncomplicated (SCOPE) 0.9798727 0.0125141 Inf 0.9556499 1.004709 0.111 ns
GDM/Uncomplicated (STOP) 0.9798727 0.0125141 Inf 0.9556499 1.004709 0.111 ns

59.6.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

59.6.6 Plots